Question

Buffer problems with additional solutions

I know this is different from normal buffer problems in that you have to add additional grams of something at the end, just not sure what to do with all the extra available solutions/solutes listed.

Outline how you would prepare 500 mL of the following solutions:

0.01M Tris, pH 7.5, 100 mM NaCl

0.05 M Tris, pH 7.8, 500 mM NaCl, 20 mM CaCl2

100 mM Tris, pH 8.7, 20 mM EDTA

You have the following available:

Tris base (FW = 121.1 g/n, pKa = 8.21)

NaCl (FW = 58.44 g/n)

100 mM CaCl2

6 M HCl

6 M NaOH

500 mM EDTA