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a protein in your cells (lactxase) converts lactate to another product lactate-x. the enazyme is specific for lactate (ch3-choh-coo-) and cannot use lactic acid ( ch3-coh-cooh) as its substrate. given the dissociation constant (Ka) is .001, the cell PH is 6.5 and glycolosis has just produced 10nM lactic acid, indicate how much lactate is available to lactxase.

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Jean Keeling
Jean KeelingLv2
29 Sep 2019

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Related questions

Just need help with a few questions from my lab!

Lab Notes:

Experiment 1:

Tube 1: No bubbles produced

Tube 2: Bubbles produced, slowly and sporadically

Tube 3: Bubbles produced, quickly and constantly

Tube 4: Bubbles produced, slowly and sporadically

Tube 5: No bubbles produced

Optimum temp for catalase activity: 40 C

Experiment 2:

Tube 1: 4 min, 42.38 sec

Tube 2: 5 min, 4.37 sec

Tube 3: 5 min, 18.26 sec

Tube 4: 5 min, 28.36 sec

Concentration: 10 mL 3% hydrogen peroxide

Experiment 3:

Tube 1: No bubbles produced

Tube 2: Bubbles produced, quickly

Tube 3: No bubbles produced

Optimum pH for catalase activity: pH 6 buffer

Lab Questions

Experiment 1:

1. Why did catalase fail to function at 80 °C?

2. What caused the bubbles to form when you added the catalase to the hydrogen peroxide and water mixture at 40 °C?

A. Catalase activity heated the solution to its boiling point.

B. Hydrogen gas formed during the formation of hydrogen peroxide.

C. Oxygen gas formed during the decomposition of hydrogen peroxide.

D. The constant temperature bath heated the solution to its boiling point.

Experiment 2

3. Before the catalase concentration was saturated with hydrogen peroxide, why did the enzyme function vary with substrate concentration?

4. Why did the catalase function level off when different hydrogen peroxide concentrations were tested?

Experiment 3

5. Why did catalase only function in a limited range of pH values?

6. Based on your results from this lab, would you expect the hydrogen peroxide you buy from the store to bubble like soda when you open it?

A. Yes, hydrogen peroxide is only stable in the presence of catalase. Without it, store bought hydrogen peroxide decomposes quickly.

B. No, catalase sped up the rate of the reaction. Store bought hydrogen peroxide will decompose into water and oxygen gas much too slowly for visible bubbles to form.

C .No, hydrogen peroxide can only decompose into water and oxygen gas in the presence of catalase.

D. Yes, catalase had little effect on hydrogen peroxide decomposition. It would bubble quickly no matter what.

7. Do the optimal conditions for catalase functioning that you found correspond with the conditions in the mammalian liver where it came from?

A. Yes, catalase worked best at a neutral pH and temperature of 40 °C, both of which are close to mammalian tissue conditions.

B. No, catalase functions best at a much higher pH than in mammalian tissue and at a much lower temperature.

C. Yes, catalase functions best at the same low pH and temperature as occurs in the mammalian tissue.

D. No, catalase functions best at several very different pHs and temperatures, while the pH and temperature of mammalian tissue does not fluctuate.

8. How much slower would hydrogen peroxide likely be decomposed if catalase was not available in cells?

A. 100 times

B. 1,000 times

C. 10 times

D. 1,000,000 times

9. What are the enzyme, substrate, and product in these experiments?

10. What role does catalase play in the mammalian liver, or any other cell it is found in?

Thank you!!

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