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19 Nov 2019
I. The molar extinction coefficient of NADH at 340 nm, ε 6220 M-1 cm-1. If I cm cuvette is used throughout experiments, how many mM of NADH are generated with an increment of 100 ab- sorbance at 340 nm? 2. In general cases, the Km of Lactate is about 10 mM, and Km of NAD is about 0.5 mM. Why the amount of both lactate and NAD were added, in this part of experiment, more than 10 times higher than their Km? 3. Why do we need to collect the baseline for the initial 30 seconds before adding any ezymes in the reaction system? For an enzyme fitting in Michaelis-Menten kinetics, what is the reaction velocity as a percentage of Vmax, when [S] = Km: [S1-0.5 Km; [S]-2 Km; [S]-|0 Km? 4.
I. The molar extinction coefficient of NADH at 340 nm, ε 6220 M-1 cm-1. If I cm cuvette is used throughout experiments, how many mM of NADH are generated with an increment of 100 ab- sorbance at 340 nm? 2. In general cases, the Km of Lactate is about 10 mM, and Km of NAD is about 0.5 mM. Why the amount of both lactate and NAD were added, in this part of experiment, more than 10 times higher than their Km? 3. Why do we need to collect the baseline for the initial 30 seconds before adding any ezymes in the reaction system? For an enzyme fitting in Michaelis-Menten kinetics, what is the reaction velocity as a percentage of Vmax, when [S] = Km: [S1-0.5 Km; [S]-2 Km; [S]-|0 Km? 4.
Bunny GreenfelderLv2
24 Apr 2019