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18 Nov 2019
1. An article (J. Agric. Food Chem. 1998, 46, 2671-2677) measured the binding constants for ANS binding to BSA and ovalbumin (OVA). In each case the reaction is ANS + protein â protein-ANS complex. K(BSA) = 6 X 106 and K(OVA) 2 X 10, ANS only fluoresces strongly when bound to protein. Set up equilibrium (K) calculations to calculate the following answer the one sig fig: You have two solutions with equal molar concentrations of BSA and OVA. You add some ANS to the BSA solution and observe a certain amount of fluorescence. How many times more grams of ANS would you have to add to the OVA solution to observe the same amount of fluorescence? (You do not need any more information to solve this problem if you assume the ratio of bound protein to free protein is similar for the two tubes when fluorescence is the same!) Compare this answer to the results you observed in lab comparing ANS fluorescence for egg white samples and BSA samples.
1. An article (J. Agric. Food Chem. 1998, 46, 2671-2677) measured the binding constants for ANS binding to BSA and ovalbumin (OVA). In each case the reaction is ANS + protein â protein-ANS complex. K(BSA) = 6 X 106 and K(OVA) 2 X 10, ANS only fluoresces strongly when bound to protein. Set up equilibrium (K) calculations to calculate the following answer the one sig fig: You have two solutions with equal molar concentrations of BSA and OVA. You add some ANS to the BSA solution and observe a certain amount of fluorescence. How many times more grams of ANS would you have to add to the OVA solution to observe the same amount of fluorescence? (You do not need any more information to solve this problem if you assume the ratio of bound protein to free protein is similar for the two tubes when fluorescence is the same!) Compare this answer to the results you observed in lab comparing ANS fluorescence for egg white samples and BSA samples.
Elin HesselLv2
3 Jul 2019