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18 Nov 2019
7. (10 points)You may recognize this one from a problem set, thus this is your lucky day. Your task is to determine the concentration ofTotal spike Detector Signal [uA] 1.78 opamine in a solution of homogenized brain tissue; 1.e. determne the |volume added concentration of dopamine in an extremely complex matrix of brain tissue that has been run through a blender to "homogenize" it; a matrimLj that is so complext that you are unable to reproduce it and thus unable to use a traditional calibration curve method. So you will utilize the method of standard additions. You have verified that your method and instrument yield a linear electrical current response that is directly proportional to the concentration of dopamine in the overall solution. You have a 50.0 mL aliquot of the brain solution to which you spike with a 0.279 nM dopamine solution Utilize the table of data and the graphical procedure for standard addition to determine the 0850 concentration of dopamine in the initial brain solution. Do this in Excel (or some spreadsheet program (or Python) and attach 1-page including the data, plot, and best-fit line equation. You do not need to consider uncertainty in this problem and report the final concentration with 3 significant figures. Make sure to recognize this data is in ml, not L 0.000 0.050 0.250 0.400 0.550 0.700 2.00 2.81 3.35 3.88 4.37 4.86 5.33 5.82 1.000 | 1.150
7. (10 points)You may recognize this one from a problem set, thus this is your lucky day. Your task is to determine the concentration ofTotal spike Detector Signal [uA] 1.78 opamine in a solution of homogenized brain tissue; 1.e. determne the |volume added concentration of dopamine in an extremely complex matrix of brain tissue that has been run through a blender to "homogenize" it; a matrimLj that is so complext that you are unable to reproduce it and thus unable to use a traditional calibration curve method. So you will utilize the method of standard additions. You have verified that your method and instrument yield a linear electrical current response that is directly proportional to the concentration of dopamine in the overall solution. You have a 50.0 mL aliquot of the brain solution to which you spike with a 0.279 nM dopamine solution Utilize the table of data and the graphical procedure for standard addition to determine the 0850 concentration of dopamine in the initial brain solution. Do this in Excel (or some spreadsheet program (or Python) and attach 1-page including the data, plot, and best-fit line equation. You do not need to consider uncertainty in this problem and report the final concentration with 3 significant figures. Make sure to recognize this data is in ml, not L 0.000 0.050 0.250 0.400 0.550 0.700 2.00 2.81 3.35 3.88 4.37 4.86 5.33 5.82 1.000 | 1.150
Reid WolffLv2
27 Apr 2019