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18 Nov 2019
ki k3 E + k2 An enzyme catalyzed reaction obeying Michealis-Menten kinetics has the following rate ki = 3.2 X 107 Mr's; k2 = 7.3 X 102 s. k 5.8 X 10s (A( 2 pts) Calculate K, for this enzyme. (B) (2 pts) Calculate Km for this enzyme. 3 (3.1x10㤠(C) (2 pts) Calculate kcat for this enzyme. (D)( 2 pts) Calculate the specificity constant for this enzyme. (EX 2 pts) If the enzyme concentration is 6.2 X 10 M, calculate Vmas for this enzyme. (F) (2 pts) If the enzyme concentration is 6.2 X 109 M, What concentration of substrate would generate a velocity equal to 0.25Vmax? (G)( 2 pts) If the enzyme concentration was increased to 8.4 X10" M, what would be the values of V max and Km?
ki k3 E + k2 An enzyme catalyzed reaction obeying Michealis-Menten kinetics has the following rate ki = 3.2 X 107 Mr's; k2 = 7.3 X 102 s. k 5.8 X 10s (A( 2 pts) Calculate K, for this enzyme. (B) (2 pts) Calculate Km for this enzyme. 3 (3.1x10㤠(C) (2 pts) Calculate kcat for this enzyme. (D)( 2 pts) Calculate the specificity constant for this enzyme. (EX 2 pts) If the enzyme concentration is 6.2 X 10 M, calculate Vmas for this enzyme. (F) (2 pts) If the enzyme concentration is 6.2 X 109 M, What concentration of substrate would generate a velocity equal to 0.25Vmax? (G)( 2 pts) If the enzyme concentration was increased to 8.4 X10" M, what would be the values of V max and Km?
Jean KeelingLv2
30 May 2019