1
answer
0
watching
556
views
17 Nov 2019
Post-Laboratory Questions You are given a sample solution that contains the same dye as you analyzed in Parts 1-3 of experiment. The concentration of a solution is unknown and needs to be determined. The absorbance of the dye was measured to be 0.130 at the wavelength used to establish the Beer's plot. Use the data you collected in this experiment to answer the following questions. a. What is the wavelength used to measure the absorbance of the unknown solution? b. Is it reasonable to use the same wavelength to measure the absorbance of this solution? Explain. c. Calculate the concentration of this solution using your Beer's Law plot? (Show all calculations/set-up used to answer this questions to receive credit.) 2. Will the following scenarios increase, decrease or have no effect on the absorbance reading of the solution? Explain your a. After the cuvette was rinsed with water, the sample to be measured was transferred to the cuvette without rinsing the cuvette with the sample solution or drying the cuvette. b. The wavelength of the instrument was unknowingly set 20 nm above nmarused for the Beer's Law plot. 14
Post-Laboratory Questions You are given a sample solution that contains the same dye as you analyzed in Parts 1-3 of experiment. The concentration of a solution is unknown and needs to be determined. The absorbance of the dye was measured to be 0.130 at the wavelength used to establish the Beer's plot. Use the data you collected in this experiment to answer the following questions. a. What is the wavelength used to measure the absorbance of the unknown solution? b. Is it reasonable to use the same wavelength to measure the absorbance of this solution? Explain. c. Calculate the concentration of this solution using your Beer's Law plot? (Show all calculations/set-up used to answer this questions to receive credit.) 2. Will the following scenarios increase, decrease or have no effect on the absorbance reading of the solution? Explain your a. After the cuvette was rinsed with water, the sample to be measured was transferred to the cuvette without rinsing the cuvette with the sample solution or drying the cuvette. b. The wavelength of the instrument was unknowingly set 20 nm above nmarused for the Beer's Law plot. 14
Jean KeelingLv2
13 Mar 2019