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16 Nov 2019
Immediate help needed
The following is lineweaver-Burke plot of an experimental result for determination of k_, k_m and V_max of an enzyme that catalyze the transformation of its substrate (S). The reaction rates were measured in unit of mu M/min and concentration of substrate is in mM, The equation shows the mathematical between1/v(Y) vs 1/(S) (x). Make sure the unit for each of the following parameter from your calculation is clearly shown. a. Calculate V_max b. Calculate K_m c. If the concentration of the enzyme in the enzymatic reaction mixture is 30 nM, Calculate k_ d. What would be the initial reaction rate if the concentration of substrate is 100 mu M
Immediate help needed
The following is lineweaver-Burke plot of an experimental result for determination of k_, k_m and V_max of an enzyme that catalyze the transformation of its substrate (S). The reaction rates were measured in unit of mu M/min and concentration of substrate is in mM, The equation shows the mathematical between1/v(Y) vs 1/(S) (x). Make sure the unit for each of the following parameter from your calculation is clearly shown. a. Calculate V_max b. Calculate K_m c. If the concentration of the enzyme in the enzymatic reaction mixture is 30 nM, Calculate k_ d. What would be the initial reaction rate if the concentration of substrate is 100 mu M
Trinidad TremblayLv2
22 Apr 2019