Question

As a technician in a large pharmaceutical research firm, you need to produce 400.mL of 1.00 Mpotassium dihydrogen phosphate buffer solution of pH = 6.82. The pKa of H2PO4? is 7.21.

You have the following supplies: 2.00 L of 1.00 M KH2PO4 stock solution, 1.50 L of 1.00 M K2HPO4 stock solution, and a carboy of pure distilled H2O.

A) How much 1.00 M KH2PO4 will you need to make this solution?'

B) If the normal physiological concentration of HCO3? is 24 mM, what is the pH of blood if PCO2drops to 22.0mmHg ?