I really need help with my Instrumentation lab report please.
Thank you
CHML 421 Chemical Instrumentation Lab Experiment 4 (Part 2 of Exp 3) Determination of Fluorescein in Antifreeze by Fluorescence Spectroscopy Fluorescence spectroscopy may be used as both a qualitative and quantitative analytical tool The observable excitation and emission spectra are characteristic of a given molecule and may be used to identify the fluorescing molecule. Emission spectra may also be used to quantitatively analyze fluorescent species. The intensity of radiated emission, keeping excitation and emission wavelengths constant, is proportional to the number of molecules in the excited state relaxing back to the ground state. This in turn is proportional to concentration of molecules in solution Where lfis the intensity of the emission radiation k is a proportionality constant for that particular cell and fluorescing species c is the concentration of the analyte in solution. Low concentrations of fluorescein yield intense fluorescence, thus concentrations in this lab are extremely low. In this experiment you will become familiar with the general use of fluorescence spectroscopy and determine the concentration of antifreeze. Chemical Hazards Fluorescein solutions are in 0.1 M NaOH, so use gloves and safety eyewear to avoid contact Laboratory Procedures Preparation of Calibration Standards 1. Prepare a 1 mg/mL (1000 ppm) fluorescein stock solution (50 mL) by dissolving 50 mg fluorescein disodium salt (MW 376 g/mol) in 0.1 M NaOH. (fnecessary prepare a second 100 ppm luorescein stock solution (50 ml) from 1000 ppm stock to prepare calibration standards.) 2. Prepare a set of 5 solutions (10 mL each) varying in concentration 02.0.5. 1.2, and 5 ppm fluorescein standard solution from 100 ppm stock solution of uorescein in 0.05 MINaOH Constructing Calibration Curves 1. Using the 2 ppm fluorescein solution obtain the excitation spectrum of fluorescein from 300 - 700 nm (select between 600 to 630 nm as emission wavelength). 2. Print out the spectrum 3. Using the same solution, obtain the emission spectrum of fluorescein from 300-700nm (select 325 nm as excitation wavelength).
