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11 Nov 2019

How to pipet 1:10 solution, draw a picture?

draw the solution color in each tube

Please label the pipet as well. For example, a 0.2ml looks like what in the pipet. Labeled

Practice SERIAL DILUTION - Equipment and Materials Needed test tube rack 6 test tubes, 12 X 75 mm pipets: 1- 2 ml 2-1 ml solution of food coloring in distilled water (1 distilled water Method 1. Label tubes 1 through 6 and place in rack. 2. Pipet 1.0 ml of distilled water (diluent) in tubes 2-6. â3. Pipet 1.8 ml of distilled water into test tube #1 .ã Pipet 0.2 ml of the peacoormosouton/into tube #1. Tube number 1 now contains twice the volume of any other tube. 4. Mix the contents of tube #1 and transfer 1.0 ml of it into tube #2. Mix contents of #2 and transfer 1 .0 ml of it into tube #3. Continue in this manner until tube #6. 6, 7. Mix the contents of tube 5 transfer 1.0 ml to tube 6. Mix the contents of tube 6 and discard 1.0 ml. 8. Measure the absorbance of each tube using the spectrophotometer at Note: The volume of each tube should be the same. Draw the serial dilution on the back. 490nm (follow the instructions)

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Sixta KovacekLv2

30 Jan 2019

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Prepare Mg standards: Dilute 100-ppm Mg standard stock solution (you made previously) to the following concentrations in the previously cleaned test tubes. Use volumetric pipets to transfer the liquids. Again this is your standard solutions whose concentration accuracy is critical to your results. Treat the pipet used to transfer the standard solution with all the proper cleaning/coating steps. Read the markings on the pipet carefully during transfers.

[Mg] made Volume of 100-ppm Mg Volume of d.i. water

(use 5-mL grd. Pipet) (use 10-mL grd. Pipet)

50 ppm 5.00 mL 5.00 mL

25 ppm 2.50 mL 7.50 mL

12.5 ppm 1.25 mL 8.75 mL

Mix the solution in each test tube well by capping and inverting the text tubes.

At this point, place another seven (7) clean test tubes in a test tube rack. Label them 1 to 7.

2. In test tube 1 pipet 0.5-mL d.i. water. In Test Tubes 2-4 pipet 0.50-mL of three Mg standards you just made, one solution to one test tube. In Test Tubes 5-7 pipet 0.5-mL of the bovine serum sample.

3. Add 2.5-mL of TCA to each test tube prepared in Step 2, cap and mix well, and let stand for 5 minutes.

4. Centrifuge all 7 test tubes for 5 minutes. Make sure that you place the test tubes in the centrifuge in a symmetrical pattern so that the spinning will not be off balance. The test tubes will break if off-balance spinning occurs.

5. Use a pipet to transfer 2.00-mL of each solution or the supernatant solution from Step 4 into another set of 7 clean test tubes. To each transferred solution, add 2.00-mL of the TY working solution you made today and 1.00-mL (with a pipet) of 2.5M NaOH solution. Cap, mix well, and let stand for 5 minutes.

6. Bring these seven solutions to a Spectronics 20D spectrophotometer. Set the monochromator to 540nm. Zero the Transmittance with nothing in the cuvet chamber and then zero the Absorbance with water in the cuvet chamber. Measure the absorbance of each of the seven solutions, standards first from the least concentrated to the most concentrated, and then the serum samples.

Based on the procedures delineated in Steps (1)-(6), draw a "flow chart" to follow the path of your analyte Mg. Establish proportional relationships between (1) ppm Mg in serum calculated from the calibration curve with that in the original serum sample; (2) ppm Mg from the calibration curve with that in the curvet. (E.g., if you calculated 10-ppm Mg off the graph, what is it in the serum sample? In the cuvet?)

The chemicals that will be used in this experiment are: 2.0 x 10-4 M KSCN Potassium thiocyanate is the source of SCN- .

0.20 M Fe(NO3)3 Iron(III) nitrate is the source of Fe3+. Clean and dry 6 large test tubes, then label them #1 - #6. Use a 5 mL Pipetman to transfer 5.00 mL of 2.0 x 10-4 M KSCN solution into each test tube.

Each of the six test tubes should have 5.00 mL of potassium thiocyanate in it at this point. Test Tube #1 To the potassium thiocyanate solution in test tube #1, add 5.00 mL of the 0.20 M Fe(NO3)3 solution.

This solution will be used as the standard; SCN- is the limiting reactant, and the large excess of Fe3+ converts all of the SCN- into FeSCN2+. This sample will have the maximum concentration of FeSCN2+ complex and the most intense color.

Test Tube #2 Pipet 10.0 mL of the 0.20 M Fe(NO3)3 solution into a 25 mL graduated cylinder. Add distilled water to get 25.0 mL of solution. Mix thoroughly.

What is [Fe3+] in this 25.0 mL solution? Add 5.0 mL of the 25.0 mL Fe3+ solution to test tube #2. Remember that the tube already contains KSCN. What is [Fe3+]o for test tube #2? What is [SCN- ]o for test tube #2? Test Tube #3 Discard all but 10.0 mL of the Fe3+ solution in the graduated cylinder. To this 10.0 mL, add water to get 25.0 mL of solution.

Mix thoroughly. What is [Fe3+] in this 25.0 mL solution? Add 5.0 mL of the 25.0 mL Fe3+ solution to test tube #3. Remember that the tube already contains KSCN. What is [Fe3+]o for test tube #3? What is [SCN- ]o for test tube #3? Test Tube #4 Again, discard all but 10.0 mL of the Fe3+ solution in the graduated cylinder.

To this 10.0 mL, add water to get 25.0 mL of solution. Mix thoroughly. What is [Fe3+] in this 25.0 mL solution? Add 5.0 mL of the 25.0 mL Fe3+ solution to test tube #4. Remember that the tube already contains KSCN. What is [Fe3+]o for test tube #4? What is [SCN- ]o for test tube #4?

A protocol tells you to prepare the following dilution: Label 5 tubes 1-5. Add 1.50 ml of diluent to each tube. To the first tube (tube #1), add 500 microliters of 1.00 M glucose and mix. Transfer 500 microliters from this first tube to the second tube (tube #2) and mix. Similarly, continue to transfer 500 microliters from each of the remaining tubes to the tube that follows it until all fivbe tubes have been used. What is the final concentration of glucose in tube #5?

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