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11 Nov 2019
Biochemistry:
4. You are studying an enzyme inhibitor, and you collect the following kinetic data on in the presence and absence of 100 μM inhibitor: Vo Cumol min) - Inh Inh 10.4 14.5 22.5 33.8 40.5 Slo (uM 10 30 90 2.1 2.9 4.5 6.8 8.1 a. Plot the data using a Lineweaver-Burk linearization (again, using a spreadsheet is recommended) b. What is the mode of inhibition? c. Where does the inhibitor bind to the enzyme relative to the substrate? d. Calculate the Ki of the inhibitor e. Propose a simple experiment to determine whether inhibition is reversible or irreversible.
Biochemistry:
4. You are studying an enzyme inhibitor, and you collect the following kinetic data on in the presence and absence of 100 μM inhibitor: Vo Cumol min) - Inh Inh 10.4 14.5 22.5 33.8 40.5 Slo (uM 10 30 90 2.1 2.9 4.5 6.8 8.1 a. Plot the data using a Lineweaver-Burk linearization (again, using a spreadsheet is recommended) b. What is the mode of inhibition? c. Where does the inhibitor bind to the enzyme relative to the substrate? d. Calculate the Ki of the inhibitor e. Propose a simple experiment to determine whether inhibition is reversible or irreversible.
Trinidad TremblayLv2
2 Jan 2019