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11 Nov 2019
Below left is a Lys-Glu-Ser tripeptide. Draw the fully protonated form of this tripeptide and the various species that would be present as you titrate with a strong base. Clearly indicate the overall charge of each. You may abbreviate the structure as shown below right. 1. 2. Sketch the titration curve for this tripeptide below. Label the axes and pK, 's. Label the charges at each 3. Estimate the pl for the tripeptide. Show calculations and justify your answer. point on the curve. 4. What two species will be present at plH -7.4? Use the Henderson-Hasselbach equation to determine a 5. NOTE: In peptides and proteins, the N-terminal and C-terminal groups have different pK,'s from the parent ratio of those two species at plH 7.4 Would the tripeptide Ser-Glu-Lys have the same pl as Lys-Glu-Ser? amino acid. N-terminal 8.5 and C-terminal -3.5. Use the pK, values from your textbook. coO OH coo
Below left is a Lys-Glu-Ser tripeptide. Draw the fully protonated form of this tripeptide and the various species that would be present as you titrate with a strong base. Clearly indicate the overall charge of each. You may abbreviate the structure as shown below right. 1. 2. Sketch the titration curve for this tripeptide below. Label the axes and pK, 's. Label the charges at each 3. Estimate the pl for the tripeptide. Show calculations and justify your answer. point on the curve. 4. What two species will be present at plH -7.4? Use the Henderson-Hasselbach equation to determine a 5. NOTE: In peptides and proteins, the N-terminal and C-terminal groups have different pK,'s from the parent ratio of those two species at plH 7.4 Would the tripeptide Ser-Glu-Lys have the same pl as Lys-Glu-Ser? amino acid. N-terminal 8.5 and C-terminal -3.5. Use the pK, values from your textbook. coO OH coo
Patrina SchowalterLv2
4 Aug 2019