The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine.
The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. T Map o abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine Enzyme Target sequence Cleavage 5' G 3' CTTAA 5' GAT ATC 3 3' CTA TAG 5 5' GG CC 3 3' CC GG 5 5' A 3' TTCGA 5' RG 3' YCCWG AATTC 3 G 5' EcoRl EcORV Haelll 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 AGCTT 3' A 5' Hindlll 3' TTCGAA 5 5' RGGWCCY 3 PpuMI GWCCY 3 GR 5 3' YCCWGGR 5 Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? a) 5' ATCGAACTAGGCC 3' b) 5' AAAGCTTGTGATATC 3 â¡ EcoRV â¡ EcoRI â¡ Hindi â¡ Haelll â¡ Haelll â¡ HindIII â¡ EcoRV EcoRI continued below... Plasmid DNA and a gene of interest are cut with the en the sticky end of the gene in the 5' to 3' direction zyme PpuMI. Write a possible sequence of bases for 5' 3'
