BCH 2333 Lecture Notes - Lecture 2: Aspartic Acid, Lysine, Carboxylic Acid
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Given the information below, which of the following statements is CORRECT?
Protein | Molecular Weight (kDa) | pI |
A | 55 | 6.0 |
B | 13 | 5.9 |
C | 57 | 9.6 |
A) Given a mixture of Protein A and Protein B, ion exchange chromatography can be used to separate the two proteins
B) Given a mixture of Protein A and Protein C, gel filtration chromatography can be used to separate the two proteins
C) Given a mixture of Protein B and Protein C, gel filtration chromatography or ion exchange chromatography could be used to separate the two proteins
D) Given a mixture of Protein B and Protein C, gel filtration chromatography can be used to separate the two proteins but ion exchange chromatography could NOT be used to separate the two proteins
A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?
options:
a) | gel-filtration chromatography | ||||||||||
b) | SDS-polyacrylamide gel electrophoresis | ||||||||||
c) | mass spectrometry | ||||||||||
d) | cation exchange chromatography | ||||||||||
e) | anion exchange chromatography 2. Consider a mixture of three peptides. The three peptides have the following primary structure: A) asp-asp-val B) glu-asp-glu C) val-val-lys Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7? options:
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