Lecture : Activity_1_-_Murder_by_the_Numbers_Full

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rescuerory02

17 Jan 2023

School

John Abbott College

Department

Biology

Course

101-NYA-05

Professor

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Document Summary

Activity #1 (part 1 - individual work) murder by the numbers. Your job is to profile each of your five murders along the following lines. Montreal murder lists can be found at the end of this document. How have you come to understand the murder and the charge, explain in a short paragraph. *the points that are underlined above, are of primary importance here. These profiles should be organized in a word doc as a list of profiles by case number. Get in touch with the homicides your group has been assigned to profile. Start getting to know some of the details of these homicides so that you will be a useful group member. Each group member is expected to profile 5 murders in montreal within the past 5-6 years. These are the murders each group is responsible for profiling. 2016: murder 15, 16, 17, 18, 19, 20. These are the links to the 2016 to 2020 montreal murders.

Related Questions

You have discovered a new Xenopus gene that codes for a RNAbinding protein. You are not sure what its function is but you areinterested in finding out as much as you can about the protein'srole in development of the embryo (from oocyte to tailbud stage) sothat you can publish your results. You have tentatively called theprotein Xp65 (Xenopus protein 65 kDa).

A) How would you go about determining where and when the gene isexpressed in the embryo? Make sure that you think about all theimportant information that you would want to know to understandwhen and where this gene product might be functioning. B) Once youhave established the expression profile, what might you want to doto help you understand how the gene effects development? Explainwhat experiments you might run, and why.

C) The protein turns out to have a role in transcriptionalactivation during gastrulation in addition to its RNA bindingactivities. What might you do to determine the targets of thisprotein (what it turns on)?

D) You find that the gene has an ortholog in Drosophila. Whatmight you want to do in Drosophila that you cannot do in Xenopus?Explain why this might work in Drosophila but not Xenopus.

mauveantelope76

please solve this:

Cancer Biology (Oncogenic Pathways)

The majority of malignant cells rely on dysregulation of a handful of oncogenes and their associated signaling pathways to support their abnormal proliferation and survival. The list below indicates the proteins that have become the âname sakeâ for these key dysregulated pathways. Note that sometimes the protein listed is mutated and drives the pathway (ex. Ras), but other times it is rarely mutated (ex. Akt where PI3K and PTEN mutations predominate).

Working together in your groups, please create a portfolio of âprofilesâ for the pathways shown below. For each pathway, include a map that links together the main proteins or molecules involved- ligands, receptors, transcription factors, etc... but always end with the ultimate effect on the cell! These pathway maps must be your own creation- do not copy other image files. For each protein or molecule you list in the map, please provide a âkeyâ that describes what it is, its specific function in the pathway, and detail where the most common pathogenic mutations occur in at least three proteins in the pathway (see below for details). Since these pathways can be extremely complex, you need not include more than three proteins or molecules at any one level in the pathway.

For information on the pathogenic mutations, use the COSMIC (catalogue of somatic muations in cancer) resource at http://cancer.sanger.ac.uk/cosmic . Enter the gene/protein name Ã click on the gene name in table below Ã under âMutation Impactâ select Pathogenic Ã Apply. A bar graph depicting the various mutations will be shown, provide the number of confirmed mutations and the changes (DNA and amino acids) it causes at that site. For example, in the BRAF gene: 24,291 mutations have been confirmed that involve a T to A mutation at base 1799 which leads to a Valine (V) to Glutamic acid (E) amino acid substitution at position 600 (note this mutation constitutively activates the BRaf kinase).

Key Oncogenic Pathways to Profile:

Ras

Akt

Wnt

TGFB

Notch

Hedgehog

Grading will be based on aesthetic clarity, thoroughness and accuracy of the information.

Grading Rubric: 75 points total

Visuals: Aesthetic clarity 30 points

Pathways are well organized, easy to follow

activating and inhibitory relationships are readily apparent

Creative layout

Content: Thoroughness and Accuracy 30 points

All pathways completed

Essential level of detail in key

A group of scientists at the Centers for Disease Control (CDC) noted 13 cases of Salmonella enterica infection in sick people in a dozen states during November 2008. The typical symptoms of salmonellosis (infection with salmonella) include vomiting and diarrhea, and may result from ingesting any of more than 1,500 different *strains, *or unique subspecies, of S. enterica. Two weeks later, a similar outbreak of 27 cases of the disease, spread across 14 states, was found to be caused by the same strain of the organism seen in the first outbreak. By February 2009, 682 people from 46 states and Canada had become infected, nine had died, a large corporation had filed for bankruptcy, and several criminal investigations had begun. Pulse Net is a branch of the CDC that seeks to identify food-borne disease clusters by carefully studying the bacterial isolates thought to be the source of an outbreak. Usually this means obtaining DNA profiles, called fingerprints*, of each bacterium and using that information to compare*isolates *(isolated strains of bacteria) from different outbreaks. Because the fingerprints from the two outbreak strains in this case were similar to one anotherâbut also different from any fingerprint within the Pulse Net databaseâCDC scientists initiated an epidemiological investigation .S. enterica was identified in unopened 5-pound containers of King Nut peanut butter in Minnesota and Connecticut, in the peanut butter factory,and in bacteria isolated from the patients. At the time, King Nut peanut butter was manufactured by the Peanut Corporation of America (PCA) in Blakely, Georgia, and sold to schools, hospitals, restaurants, cafeterias, and other large institutions rather than directly to consumers. Examination of the bacteria revealed several different S. enterica strains, but only a few of them were linked to the illnesses.

Without knowing the specific details of DNA fingerprinting ,how do you think these profilescould be used to show that a particular bacterial strain is not part of an outbreak?

Lecture : Activity_1_-_Murder_by_the_Numbers_Full

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