BS 375 Lecture Notes - Papain, Immunoglobulin Heavy Chain, Immunoglobulin Light Chain

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wrygaard6

15 Jan 2023

School

University of the Sciences

Department

Biological Sciences

Course

BS 375

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Document Summary

Immunoassays are analytical methods used for detection and quantification of the antigen-antibody interaction. Antigen: any compound or substance which induces the production of antibodies in warm-blooded animals. Antibody: y-shaped complex protein produced in response to the antigen by the immune system by b-cells. Antibody molecule has 4-polypeptide chains: two identical heavy chains (h-chains, two identical light chains (l-chains) These chains are joined together by disulfide linkages to form y shaped structure. Heavy chain consists of 400 amino acids, while light chain has 200 amino acids. Within each polypeptide chain there are two regions: constant region, variable region. The arms of the antibody are identical, and a single molecule of antibody may combine with two antigens to form antibody-antigen complex. The antibody molecule can be broken down into two parts with an enzyme papain : The first antibody to appear in the circulation by b-cells. About 10% of total ab-component of normal serum.

Related Questions

Question 1

The antigen specificity of a BCR...

A.	depends on the isotype of that BCR.

B.	is the same as the antigen specificity of the secreted form of antibody produced by that cell.

C.	is different for each of the BCRs on a cell.

D.	is different for each of the Fab regions of the BCR.

Question 2

All BCRs on the surface of a B cell in a lymph node...

A.	will have the same type of light chain (kappa/lambda).

B.	will have the same type of light chain with the same exact amino acid sequence.

C.	will each have a different type of light chain.

D.	will have a lambda light chain on one Fab and a kappa light chain on the other Fab.

Question 3

The isotype of an antibody is...

A.	determined by the light chain.

B.	another word for an immunoglobulin Fab region.

C.	determined by the heavy chain constant region.

D.	determined by the Fc portion.

E.	None of the above are correct

Question 4

Characteristics of lgG are...

A.	a molecular weight of 900,000 and 10 antigen binding sites in the secreted form.

B.	165,000 molecular weight in the monomeric form but it is usually secreted as dimer.

C.	the presence of more heavy chain constant regions than most antibodies but the lowest concentration in blood.

D.	150,000 molecular weight and the highest concentrations of all antibodies in blood.

E.	None of the above describe IgG.

Question 5

Characteristics of lgM are...

A.	900,000 molecular weight and the secreted form has 10 antigen binding sites.

B.	165,000 molecular weight in the monomeric form but it is usually secreted as dimer.

C	.the presence of more heavy chain constant regions than most antibodies but the lowest concentration in blood.

D.	150,000 molecular weight and the highest concentrations of all antibodies in blood.

E.	None of the above describe IgM.

Select the true statements regarding antibody (specifically, immunoglobulin G) structure. Both the Fab and Fc fragments contain constant domains. Both the Fab and Fc fragments have variable domains. The antigen-binding site is formed as a combination of variable domains from one heavy and one light chain. The antigen-binding site is located in the Fc fragment. The heavy and light chains are linked by noncovalent and disulfide bonds.

While comparing two different protein-sequencing methods requiring minute amounts of protein, you established the amino acid sequence of the entire light chain of a purified immunoglobulin. From this sequence you will be able to determine [complete the sentence and assess the resulting statements (1-6) as either factually correct (= true) or incorrect (= false)]:

1. the isotype of the immunoglobulin.

2. whether the B cell that produced this antibody received T-cell help.

3. the specific variable gene fragment used to produce this chain.

4. the antigen specificity of the immunoglobulin.

5. the approximated strength of the immunoglobulinâs binding to different Fc receptors.

6. the molecular weight of the post-translationally unmodified light chain.

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BS 375 Lecture Notes - Papain, Immunoglobulin Heavy Chain, Immunoglobulin Light Chain

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