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Question 23 of 36 Map MHE/Freeman Leaming Nelson | Cox SEVEN presented by Sapling For her undergraduate project, Jessica studied an enzyme that catalyzes the reaction A For substrate A, she determined that Km 3.0 M and kcat 20 min1. Jessica graduated and her project has been passed on to you. Unfortunately, Jessica was so busy that she sometimes forgot to record all of the details of an assay in her lab notebook. Your mentor suggests that you try to back calculate some of the missing concentration values. Assume that the enzyme follows Michaelis-Menten kinetics. In one experiment where [A] = 4 mM , she found that the initial velocity, Vo was 624 nM min1 What was the [E] she used in this experiment? (b) In another experiment with Ed = 0.2 AM, she found that Vo = 3 AM min-1. What was the [A] she used in this experiment? a) Number Number E,- 31.1 nM The compound Z was found to be a very strong competitive inhibitor of the enzyme, with an α of 10. In an experiment with the same [E] as in part (a), but a different [A], an amount of Z is added that reduces the rate Vo to 312 nM min- What was the [A] in this experiment? enzyme, with an a of 10. (d) A measure of the cata enzyme is the initial slope of the Michaelis- Menten curve at low [S], the second order rate constant kcat/Km A "perfect" enzyme, limited only by diffusion, would have a value of 108 or 10° M-'s-1 Calculate Kcal/Km for this enaame. Number Number at6.66 KM Previous Give Up & View Solution Check Answer Next Exit