BSCI170 Lecture Notes - Antonie Van Leeuwenhoek, Differential Centrifugation, Robert Hooke

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kkuroda

10 Jan 2023

School

University of Maryland

Department

Biology

Course

BSCI170

Professor

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Document Summary

Studying cells: cells were discovered in late 1600s, robert hooke. Antony van leeuwenhoek: early microscope, animalcules (bacteria) Modern light microscope: typical microscope, used in high school. Electron microscope: uses a beam of electrons rather than a beam of visible light, much higher quality magnification than light microscopes. Simplifying cell complexity: structural complexity is a property of all living organisms, question, how can we study individual cellular components, answer, break the cell open, separate, isolate, and enrich components of interest. Differential cell fractionation: tissue cells homogenization homogenate differential centrifugation. Eukaryotes vs. prokaryotes: prokaryotes vastly outnumber eukaryotes, they live in every conceivable environment or ecological niche, they have limited morphologies but diverse metabolisms. Eukaryotes vs. prokaryotes: eukaryotes: more morphologically diverse than prokaryotes, the most fundamental difference is the presence or absence of a membrane-bound nucleus that stores and protects the genetic material.

Related Questions

26. Which of the following is the best microscope to use to visualize a cross section of skin
stained so that the cells are purple and the extracellular matrix is pink:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
27. Which of the following is the best microscope to use in order to visualize cell to cell
junctions at high magnification:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
28. A student is studying the tensile strength of a new metallic alloy. He wants to visualize
the fracture surfaces following tensile testing. Which is the best microscope for this
application:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
29. A graduate student is studying the degradation properties of a new polymer that she
developed. She adds a fluorescent molecule to the polymer backbone and makes a thin
layer of the polymer on a glass slide. In order to visualize the polymer and measure its
degradation/disappearance over time, which is the best microscope to use:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
30. Endothelial cells were exposed to experimental conditions and are imaged intact under a
microscope following antibody labeling of NF-ÎºB, a protein located inside endothelial
cells. Select the following statement that best describes the approach that should be
used to image the endothelial cells in this experiment.
a. Cells are imaged under a transmission electron microscope.
b. Cells are imaged using phase-contrast in the light microscope.
c. Cells are imaged under a fluorescence microscope.
d. Cells are imaged under a scanning electron microscope.

1.Of the following which would you select as the best to observe the changes in the shape of red blood cells?

		a.Scanning electron microscope
		b.Transmission electron microscope
		c.Light microscope
		d.Dissecting microscope
		e.None of the above. Cell fractionation would be the best technique to study this process

What is the major difference in scanning electron microscopy(SEM) when compared to light microscopy (LM) or transmissionelectron microscopy (TEM)?

	SEM is not good at viewing higher magnifications.
	The original image is in black and white.
	SEM uses whole, unsectioned surfaces that are covered in carbonand gold dust.
	SEM uses a beam of light to view specimens.

BSCI170 Lecture Notes - Antonie Van Leeuwenhoek, Differential Centrifugation, Robert Hooke

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