CHM-233 Lecture Notes - Lecture 4: Column Chromatography, Ninhydrin, Fluorescence
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Additionally, there are plates on the market that are coated with a fluorescent substance; under ultraviolet light, compounds that do not fluoresce but do absorb ultraviolet light appear as dark spots. 2. several reagents, including sulfuric acid, potassium permanganate, phosphomolybdic acid, and ninhydrin, can be sprayed onto the chromatographic plate;these reagents cause colored or dark spots when they interact with the individual components. 2. check tlc (thin-layer chromatography) or cc (column chromatography) as the more appropriate answer to the following questions or statements. Tlc is a quicker procedure for separating components of a mixture. Cc is better for separating a 5-gram mixture of components. Cc is better for separating a mixture of volatile compounds. The solvent will keep flowing up the plate while carrying the samples with it, the samples will start to get pushed together, until they become indistinguishable again. 4. the separating power, or activity, of a tlc plate is increased by heating the plate in an oven at 100 c.
