MEDICAL TECHNOLOGY Lecture Notes - Lecture 1: Polyacrylamide Gel Electrophoresis, Dot Blot, Western Blot
28 Oct 2022
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Answer the following questions: differentiate nucleases from polymerases. Nucleases are an enzyme capable of breaking dna molecules by cleaving the phosphodiester bonds between one nucleotide and another. Polymerases are enzymes capable of producing new strand of dna from an existing strand of dna or rna: describe the applications of nucleic acid hybridization. Southern hybridization detection of a particular dna sequence from a mixture of dna. Northern hybridization detection of a particular rna sequence from a mixture of rna. Array-based hybridization: dot blot and slot blot hybridization multiple samples are being processed simultaneously. Western blot detects a particular protein from a mixture of protein. It is based on the principle of immunochromatography wherein protein molecules are separated in polyacrylamide gel according to the molecular weight.
