BIOLOGY Lecture Notes - Lac Operon, Galactoside, Permease

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7 Sep 2022
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Detailed explanation of lac operon experiment, when the bacteria were placed in dropper bottles containing glycerol as a carbon source. In bacteria, the operon is the coordinated unit of genetic expression and is the functioning unit of dna containing cluster of genes under the control of a single promoter. In 1961, jacob and monod introduced the concept of operon and based on their observations on the regulation of lactose metabolism in e. coli, it was popularly known as. The lac operon consists of a regulatory gene (i ) for inhibition, operator gene (o), and 3. Besides these genes there is a promoter site (p), next to the operator gene where the structural genes (z,y,a). enzyme rna polymerase binds. The structural genes z, y and a respectively code for the enzymes - galactosidase, galactoside permease and galactoside acetylase. The - galactosidase hydrolyses lactose i. e. , - galactosidase to galactose and glucose. The permease is responsible for the transport of lactose into the cell.

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