Lecture : Experiment No 11

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To perform quadrant streak method for isolation of pure cultures. The techniques commonly used for isolation by discrete colonies initially require that the number of organism in the inoculum be reduced. The resulting diminution of the following inoculation, individual cells will be sufficiently for on apart on the surface of the agar medium to separate the different species. The following are the techniques that can be used to accomplish the necessary pure culture isolation. The steel plate is a rapid qualitative isolation method. It is essentially a dilution that spreading a loopful of culture over the surface of the agar plate. The sample or the inoculum is diluted boo streaking it surface of agar plates. While streaking in successive area of plates, the inoculum is diluted into the point where there is only one bacterial cells deposited every few millimeters on the surface of the agar plates.

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