CSB329H1 Lecture Notes - Macular Degeneration, Embryonic Stem Cell, International Practical Shooting Confederation
Document Summary
Lecture 5 nuclear reprogramming in eggs - first example in cloning. Human escs first derived in 1998 after mouse, needed to learn primate escs. Made in the us when federal grant money could not be used. Made using similar culture practice to mouse escs (mouse feeder cells and bovine serum - growth factors). Took longer because of ethical issues associated with using human embryos. Resemble post-implantation mouse embryo, can maintain with same media conditions. Regulatory control by funding bodies and government. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by. 4 yamanaka factors (tf) can drive fibroblast cell back to ipsc. Oct4,sox2, klf4, c-myc (2 of them part of enhanceosome). Should be derived with use of animal products. Clean facility, where there are no pathogens. Genetic alterations occurring in these cells during the derivation process is concerning. Blastocyst outgrowth assay division - dna damage. Use whole genome sequences to reveal any genetic changes that cause disease.
