Biochemistry 2280A Lecture Notes - Dna Profiling, Escherichia Coli, Synthetic Biology
19 Mar 2022
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Document Summary
2018-2019: recombinant dna technology: techniques that recombine dna fragments from different sources into new molecules w/ unique features. Significance of rdt: research: understand cell and molecular structure/function, biotechnology: major impact on society, medicine: drug production/design (ex. Gmos are controversial, no adverse health effects have been found. Production of novel molecules in plants (therapeutic drugs: manufacturing: novel products, more efficiently (detergents, spider silk, biofuels, environment: bioremediation (bacteria that consume oil, pcb, styrofoam, forensics and law: dna fingerprinting - csi. Case study: your goal is to mass produce a human protein (yfp) using rdt, why is expression of yfp using rdt important, many proteins are very difficult to obtain from their native source. Intrinsic features: about the molecule itself: g-c rich dna- more h bonding and base stacking so higher tm, 4 degrees for every g-c and 2 degrees for every a-t. Southern blot: dna probe to dna on membrane: hybridize dna to dna.
