CHEM 271 Lecture Notes - Lecture 2: Standard Error, Ammonium Sulfate, Bradford Protein Assay
Document Summary
Percentage of difference = (a-b)/ [(a+b)/2] x 100. Percentage of difference = (5. 2-4. 83)/ [(5. 2+4. 83)/2] x 100. Reason for the difference between the actual ph and observed ph. Due to long exposure to room temperatures or cross contamination between precision pipetting it is possible that the buffer solution varies from the actual ph. Where the concentration of the bsa is 2. 0 mg/ml. Absorbance vs [bsa] y = 49. 415x + 0. 2628. Absorbance vs [ldh] y = 87. 847x + 0. 0416. [ldh] mg/ml in stock solution for each sample. Example calculation using ldh sample 1 absorbance & bsa linear regression. Bsa linear regression: y = 49. 415x + 0. 2628. 0. 7522 = 49. 415x + 0. 2628 x = 0. 0099 (mg/ml) Concentration of ldh sample 1 = 0. 0099 (mg/ml) Using data sample values outside the curve will not be helpful because we do not have standards to compare the range to. Extending the curve theoretically will not yield accurate results.
