BIOLOGY 2C03 Chapter Notes -Phototroph, Wild Type, Chromosome
Document Summary
Bacterial culture and growth: binary fission, colony formation. Different microbial species have different growth reqirements, within each species: minimal medium: phototroph (wild-type strains, supplemented minimal medium: auxotrophs (specific nutrient requirements, complete medium: all strains can grow. Replica plating on different media for genotype identification. Characteristics of bacterial genomes: usually a single, circular chromosome. Plasmid can be modified and used in a variety of recombinant dna applications: high-copy-number plasmid, low-copy-number plasmid. Conjugation: transfer of replicated dna from a donor cell to a recipient cell. Transduction: transfer of dna from one bacterium to another by a viral vector. Bacteria transfer genes by conjugation through fertility (f) plasmid. The f factor contains four insertion sequence (is) elements. Is elements shared by an f plasmid and a bacterial chromosome allow for recombination between the two. Converting f+ to high frequency recombination (hfr) strains. When intact f plasmid + some host dna excised from hfr; f" cells.
