BIOL 261 Chapter Notes - Chapter Essay 4: Biological Engineering, Growth Medium, Transfer Rna
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You are a biological engineer who is working on expanding the genetic code by recoding e. coli to use an unnatural/non-standard amino acid for uag instead of stop. Briefly explain the 4 main pre-requisites in order to do so. The unnatural amino acid is first synthesized chemically in its optically pure l-form. Taylor pp, pantaleone dp, senkpeil rf, fotheringham ig. Novel biosynthetic approaches to the production of unnatural amino acids using transaminases. Metabolic pathways and fermentative production of l-aspartate family amino acids.
