B PHARMACY Lecture Notes - Lecture 12: Jamia Hamdard, Pharmacognosy, Chloramphenicol
26 Jul 2021
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Presented by: varsha srivastav: pharm 2nd semester (pharmacognosy) Insertion of copies of a gene into living cells in order to induce synthes is of the gene"s product. To add a desired trait to a crop, a foreign gene (transgene) encoding the trait must be inserted into plant cells, along with a cassette of additional genetic material. Agrobacterium tumefaciens mediated gene transfer: tumefaciens is basically a bacterium which infects wounded or damaged plant tissues, which induces the formation of a plant tumor called crown gall. Phenolic compounds acetosyringone, hydroxyacetosyringone)released by the wounded sites. Crown gall formation occurs when the bacterium releases its ti plasmid (tumor- inducing plasmid) into the plant cell cytoplasm. A fragment (segment) of ti plasmid, referred to as t-dna, is actually transferred from the bacterium into the host where it gets integrated into the plant cell chromosome (i. e. host genome).
