BIOL 2104 Lecture Notes - Lecture 2: Antigen, Centromere, Fluorophore
13 Jan 2018
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Document Summary
Module 2: tools in cell biology, tools in biochemistry, model organisms, concepts in compartmentalization. A sense of scale between living cells and atoms: cells are around 20-50 micrometers, tissue 0. 2mm, you can visualize details within the mitochondria, look at protein synthesis. What can we see: minimum resolvable by unaided eye (0. 2 mm, minimum resolvable by light microscope (200 nm, minimum resolvable by electron microscope (0. 2 nm) Inference between light waves: when waves are in phase, bright signales, waves interfere (out of phase, dim, out of color. If you want more better resolution in terms of structure inside then you need to use a different strategy. Sample processing: take individual tissues and put together and then stain it and it is mounted under a glass cover slip, resolution depends on the quality of the light source. Some molecules can be localized in cells by fluorescence microscopy: some dyes are specific.
