CHEM 103 Lecture Notes - Lecture 4: Mass Spectrometry, Food Contaminant, Natural Abundance
Document Summary
Use to find atomic mass and natural abundance. If two particles have the same charge: The angle of deflection depends on the mass to the ration (m/z) We use ms to measure masses accurately. Determine protein structure, function, folding and interactions. Identify a protein from the mass of its peptide fragments. Detect specific post-translational modifications and protein digestion. Quantitate (relative or absolute) proteins in a given sample. Monitor enzyme reactions, chemical modifications and protein digestion. Perform forensic analyses such as confirmation of drugs abuse. Detect disease biomarkers (e. g. , newborn screened for metabolic diseases) Break all atom-atom interactions to give atomic ions. Mass spectrometer drawing will have two carbon and one oxygen. Usually formed by combination of non-metals with other nonmetal; some involve metalloids or certain metals. Typically formed by combination of reactive metals with reactive nonmetals. Consists of ions packed in crystal lattice.
