BIOL 2100 Lecture Notes - Lecture 31: Growth Factor, Separase, Lamin
Document Summary
G1 phase is the phase when cells just function normally. G0 phase is when cells just exit the m phase. Important so that the chromosomes can separate efficiently. Prometaphase = break down of nuclear membrane, microtubules attach to kinetochores. At metaphase, two homologs of chromosomes do not pair up. Chromosomes need to decondense to become accessible for gene expression. When two cells at different phases were fused together, both cells synchronized to the same phase. Microinjection experiments = draw out cytoplasm of one cell into another cell. Factors that regulate the cell cycle occur in the cytoplasm. You can separate the cytoplasm by fractions based on certain factors, and test each fraction. Frog cells were used in microinjection experiments because they were easy to see and large. Mpf acts as a kinase and is a complex of two proteins. Mpf accumulates in the cell from g1 to g2, but drops during the m phase.