BIOL 483 Lecture Notes - Lecture 91: Heterochromatin, Background Noise, Nummelan Palloseura
Document Summary
Nucleoslus associated domains = nads, repressed regions, nucleolus is dense chromatin in center of nucleus. Transcription facotries = lots of gene expression occuring, activating. Nuclear speckles = rna processing bodies, activating, because genes are being transcribed into rna. Lamin associated domains (lads) = localised at nuclear periphery, typically enriched in repressive marks/ drive gene silencing. Which genomic loci are interacting with each other/ with protein of interest in nucleus. General flow: cross link chromatin the way it"s arranged in nucleus, digest, ligate ends, reverse the crosslinking, detection. Lots of different graphics throughout the lecture slides and it depends what works best for you. Chromatin that will be cross linked/digested gets cut into x - can do proximituy ligation or mark with biotin (hic) or immunoprecipitation to look for portein that interacts with these regions. Chromatin conformation capture - 3c: cross link, digest, ligate, reverse.
