FSCN 4613 Lecture Notes - Lecture 5: Petroleum Ether, Silica Gel, Elution
7 Mar 2020
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A mobile phase passes over a stationary phase and compounds are carried along with the mobile phase at rates dependent upon the relative affinity of each compound for the mobile and stationary phases. A liquid (as in thin layer chromatography) A solid (as in thin layer chromatography) Major lipid classes, from most hydrophobic to most hydrophilic, are: Thus, the order of these lipids will be as shown above, from top to bottom of the plate. You will begin by practicing spotting onto a tlc plate. Take up a capillary tube full of petroleum ether. Slowly apply it to the plate by touching the tip of the capillary tube onto the plate and slightly releasing your finger from the top of the capillary tube. You will add 200 microliters of petroleum ether to your two vials containing your lipid extracts and mix well by vortexing. Take up the petroleum in the capillary tube. Apply lipid extract to the tlc plate.
