FSCN 2021 Lecture Notes - Lecture 9: Microbiological Culture, Freeze-Drying, Norovirus
24 Feb 2019
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Contain chemicals (thioglycolate or oxyrase) that combine o2. Candle jar (use fire and the flame will distinguish without o2 that will generate co2) Make it easy to distinguish colonies of different microbes. Blood agar: a differential medium containing red blood cells. Suppress unwanted microbes and encourage desired microbes. Assume a soil sample contains a few phenol-degrading bacteria and thousands of other bacteria. Inoculate phenol-containing culture medium with the soil, and incubate. Transfer 1 ml to another flask of the phenol medium, and incubate. Trasnfer 1 ml to another flask of the phenol medium and incubate. A pure culture contains only one species or strain. A colony is a population of cells arising from a single cell or spore or from a group of attached cells. A colony is often called a colony-forming unit (cfu) The streak plate method is used to isolate pure cultures. Deep freezing: -50 degrees c to -95 degrees c.
