BIOLOGY 151 Lecture Notes - Lecture 1: Peptide, Genetic Code
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For the following strand of DNA, use the table below to provide: bases for the complementary mRNA and the associated amino acids. What do you notice about the genetic code for proline in the peptide? How would the loss of the first G or switching that G to a T affect the structure and function of this protein?
DNA: GGC CTA CAA GCC GGA ACA GTT ACC GCG TAA CGA
RNA: ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
Amino Acid:??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
(3 Letters)
Genetic Code Table (mRNA)
1st position (5' end) | 2nd position (middle) | 3rd position (3' end) | |||
U | C | A | G | ||
U | Phe F Phe F Leu L Leu L | Ser S Ser S Ser S Ser S | Tyr Y Tyr Y STOP STOP | Cys C Cys C STOP Trp W | U C A G |
C | Leu L Leu L Leu L Leu L | Pro P Pro P Pro P Pro P | His H His H Gln Q Gln Q | Arg R Arg R Arg R Arg R | U C A G |
A | Ile I Ile I Ile I Met M | Thr T Thr T Thr T Thr T | Asn N Asn N Lys K Lys K | Ser S Ser S Arg R Arg R | U C A G |
G | Val V Val V Val V Val V | Ala A Ala A Ala A Ala A | Asp D Asp D Glu E Glu E | Gly G Gly G Gly G Gly G | U C A G |
The following is a code for a strand ofDNA
DNA 3' | A | T | A | T | T | T | T | ||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |
mRNA | C | G | U | U | G | A | |||||||||||||
tRNA | U | G | G |
Amino Acid (Met __________________ _________________________________ _________________
a. Using the genetic code provided, fill in the blanks tocomplete the segment of DNA shown.
b. Fill in the blanks to complete the sequence of amino acidscoded for by this strand of DNA.
c. Write the code for the complementary strand of DNA completedin part (a).
d. What would be the effect if C were substituted for T at base10?
e. What would be the effect if A were substituted for G at base11?
f. What would be the effect if G were substituted for T at base14?
g. What would be the effect if C were inserted between bases 9and 10?
h. How would UV radiation affect this strand of DNA?