MCDB 423 Lecture Notes - Lecture 10: Hemoglobin, Wild Type, Cooperative Binding
23 Dec 2020
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On clamp t, go to le and open data. Right click on the second signal (that shows that you"re clamping the cell at -50 mv). Move cursor # 1 to the baseline and click on the check mark. Go to window, results and copy y delta 1-2. For y select enter and plot a single y value. Enter all atp concentrations (1, there is no log of 0), 2, 5, 10, 20, 50, 100, 200 um. Paste y delat 1-2, assume that 0 atp (ors) contains traces of atp and generates 0 current response. Select log (agonist) vs variable slope four parameters. For this particular example ec50 is 17. 97 and the hill c. is 1. 748. How to connect your cable for a voltage clamp recording using oocyte clamp oc-725c: Analog in 0 (front digitizer)________i monitor (front ampli er) (to read current on the current electrode)
