MCDB 423 Lecture Notes - Lecture 2: Cell Nucleus, Pipette
9 Oct 2020
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If double sanded dna: you need to choose that one (enter) If you were to measure amount of rna (you have to hit rna) Reading concentration of dna in sample in tangrams. Gonna be diluted sample 100 have to tell machine that dilution factor. Dilution factor is 1 dna sample in 99 ul of water. Avoid touching clear sides > light goes through clear sides, don"t make dirty. P10 pipette set to 1 ul of dna. Mix it going up and down up and down with pipette. Don"t want to create a lot of air bubbles. Blank machines with 100 ul of water (same water you use to dilute sample with) Place arrow and opaque side to the right of spectrophotometer. You want to get a reading to tell you if you need. a blank or sample next. Take out blank and place sample in meter. You should get a reading ex 411. 5 nangorams per micrometer.
