MCDB 423 Lecture Notes - Lecture 26: Brain-Derived Neurotrophic Factor, Apoptosis, Antibody

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 423
• Professor: John Kuwada

Document Summary

There is something about the limb bud that inhibits cell death. The final result was that the limb buds were secreting neurotrophins that prevented cell death. Removing a limb bud leads to increased cell death in the drgs that innervate it. First, to generate growing cells, she used sarcomas which are tumor cells. She implanted sarcoma cells into the chick embryo. She observed that close to where the sarcoma was placed, you would see enlarged srg and. So she put the sar(cid:272)o(cid:373)a outside the (cid:272)horioaloti(cid:272) (cid:373)e(cid:373)(cid:271)ra(cid:374)e (cid:894)so that it(cid:859)s (cid:374)ot ph(cid:455)si(cid:272)all(cid:455) tou(cid:272)hi(cid:374)g the membrane) So the sarcoma is secreting something that is keeping the neurons alive. Implant a mouse sarcoma into the chick embryo (logic was to generate a large amount of (cid:858)target(cid:859) tissue(cid:895) Animals with the implanted tumor had more neurons. The chick embryo has more neurons slide 3. Initial experiment was to do in vitro assays.