MCDB 310 Lecture Notes - Lecture 19: Glutamine Synthetase, Nitrogen Fixation, Glutamine

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 310
• Professor: Kenneth Balazovich

Document Summary

Mcdb lecture 19: amino acid & nucleotide synthesis (ch. We (human metabolism) can"t synthesize all the amino acids. Of the ones we can synthesize, only 3 precursors: notice examples of general principles. Cyclic processes of plants need more n in the soil to help other plants latet: this is a metabolically expensive process. Takes 16 atp to transform a nitrogen molecule of. Regulation of glutamine synthetase (in bacteria: gln: central entry point for reduced nitrogen into most metabolic pathways, highly regulated, two levels of regulation of glutamine synthetase: allosteric and covalent, 1. Allosteric inhibition is additive: lots of feedback inhibition by lots of different molecules that regulate glutamine synthetase, all these molecules contain nitrogen, these inhibitors take effect in an additive way, 2. Production of sulfide: comes from environment sulfates, sulfate (so4 2-) is activated by atp, reduced using 8 electrons producing sulfide (s2-, 2. In we feed bacteria 15 nh4, they incorporate this into all their amino acids.