BIOLOGY 207 Lecture Notes - Lecture 34: Light-Independent Reactions, American Society For Microbiology, Cytochrome

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15 May 2021

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Classes of microbes according to fueling reactions / energy. Will use either or both of these images at different points in the lectures. They are conceptually equivalent: within a single microbial cell. Organic substrates outside of the cell (growth media/ environment of the cell has organic molecules in it) The organic molecules will be transported inside the cell and then acted on broken down, and fed into (1 or more) of the 3 central pathways. Outside cell inside the cell central pathways generate precursor metabolites and generate energy and reducing power. Reducing power shuttled over to the electron transport system that is on the membrane. Electron transport system is going to drive h ions out of cell, creating an energized membrane (large membrane differential in energy from inside and outside cell) The movement of the protons back across membrane will drive the generation of the high phosphate bonds converting adp to atp.

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Microbiology Unit 2 Applied and Environmental Assignment Part 1: Identify Nutritional requirements from Chapter 6&14 (and review from chapters 1 & 2) 1. Determine which of the 4 macromolecules require the following elements: a. Carbon: carbohydrates___ lipids___ proteins___ nucleic acids___ b. Nitrogen: carbohydrates___ lipids___ proteins___ nucleic acids___ c. Sulfur: carbohydrates___ lipids___ proteins___ nucleic acids___ 2. The essential elements mentioned above are cycled through cells and the earth in biogeochemical cycles (the carbon, nitrogen, and sulfur cycles.)Select the correct answer or fill in the blanks to summarize these cycles: a. Carbon dioxide is taken in from the atmosphere by [autotrophs/heterotrophs] and converted to glucose through carbon ________________. When heterotrophs eat autotrophs they use catabolism to break down glucose to make ATP and waste carbon is released as ___________. b. There is plenty of nitrogen available in the atmosphere but it is not accessible to organisms because it is in the form of Nitrogen gas (N2). N2 is fixed by [bacteria/plants/animals] and converted to ammonia (NH3.) NH3 can be used by [bacteria/plants/animals] to build their cells. Next, by consuming food with amino acids,[bacteria/plants/animals] can get access to nitrogen for their own cellular needs. c. There is also a good quantity of sulfur in dirt, rocks, and the atmosphere. Sulfur cycles between H2S => S0 => SO4 => SH groups in Proteins => back to H2S.Using _________________ respiration, anoxygenic photosynthesis, and chemolithoautotrophy [bacteria/plants/animals]convert H2S and S0 to SO4 which is taken in by [bacteria/plants/animals] and used to build their cells. When [bacteria/plants/animals]eat plants they take in essential amino acids containing sulfur and recycle these to make their proteins. d. _________________ break down waste and dead organisms and recycle nutrients back into the cycles. *The C and N are summarized here: https://youtu.be/2D7hZpIYlCA and https://youtu.be/leHy-Y_8nRs 3. Match the main metabolic classifications to the description: Classifications: photoautotroph/ photoheterotroph/ chemoautotroph/ chemoheterotroph o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from sunlight o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from reduced inorganic chemicals o A microbe that eats other organisms to get organic carbon and energy o A microbe that eats other organisms to get organic carbon but gets energy from sunlight 4. Identify the phases of the bacterial growth curve and define what occurs in each phase Phase A = ______________ Definition: Phase B = ______________ Definition: Phase C = ______________ Definition: Phase D = ______________ Definition: Part 2: Unique ecosystems containing microbes 5. Watch the video that shows hydrothermal vents: https://youtu.be/RO3nO98gH_M a. Describe bacteria that live at the vents using terms from the unit 2 reading guide: ________________________________________________________________________ b. Write a 2-3 sentence reflection about hydrothermal vents and the contribution of microbes to this ecosystem: 6. Apply what you have learned to the TED-Ed video about Biofilms: https://youtu.be/pHLP5CZMnL4 a. Give the terms for cell structures mentioned in the video that we learned in Chapter 4 and 7: i. âsticky appendagesâ thatallows bacteria to attach and begin the biofilm: _______________ ii. Extracellular matrix/gooey matrix that holds cells together: ____________________________ iii. Exchange genetic information while packed together: ____________________________ iv. Communicate with each other by _________________________________________ sensing. v. Produce chemical to kill microbial competitors: ____________________________ factor.

The complete set of reactions carried out by an organism is called:

a. anabolism

b. catabolism

c. metabolism

Value: 2

The breakdown of starch is broken down to glucose is an example of:

a. anabolism

b. catabolism

c. metabolism

Value: 3

For the following statement, determine which description best describes the process: DNA is synthesized from free nucleotides.

a. anabolism

b. catabolism

c. metabolism

Value: 4

Fill in the blank: Catabolic reactions result in the synthesis of molecules of .............via cellular respiration. This is then used to power anabolic reactions.

Value: 5

The energy in organic molecules is released in a series of steps because:

a. it is not possible to release it in a single step.

b. more total energy is released in multiple steps than would be released in a single step

c. less total energy is released in multiple steps than would be released in a single step.

d. more energy can be harvested for cellular use in multiple steps than from a single step.

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photosynthesis

1-The light reaction of photosynthesis does not include

a. chemiosmosis

b. oxygen liberation

c. glucose synthesis

d. electron transport

Value: 2

Photosynthesis takes place in the membranes of small sacs called

a. thylakoids

b. photosystems

c. stroma

d. photons

Value: 3

During what stage of photosynthesis is O2 produced?

a. the light-dependent reactions involving photosystems I and II

b. carbon fixation

c. the Krebs cycle

d. O2 is not produced during photosynthesis

Value: 4

The pigment molecules responsible for photosynthesis are located in the

a. mitochondria

b. cytoplasm of the cell

c. stroma of the chloroplast

d. thylakoid membrane of the chloroplast

Value: 5

During what stage of photosynthesis are ATP and NADPH converted to ADP + Pi and NADP+?

a. the light-dependent reactions

b. the light-independent reactions

c. both of the above

d. none of the above

Value: 6

What energy-rich organic compound is produced as a result of the Calvin cycle?

a. ATP

b. NADPH

c. glucose

d. H2O

Value: 7

During photosynthesis, photons raise electrons to higher energy levels. These excited electrons belong to what compound?

a. water

b. ATP

c. chlorophyll

d. electron transport chain

Value: 8

Which of the following statements accurately describes the relationship between photosynthesis and cellular respiration?

a. photosynthesis occurs only in autotrophs; cellular respiration occurs only in heterotrophs

b. photosynthesis uses solar energy to convert inorganics to energy-rich organics; respiration breaks down energy-rich organics to synthesize ATP

c. photosynthesis involves the oxidation of glucose; respiration involves the reduction of CO2

d. the primary function of photosynthesis is to use solar energy to synthesize ATP; the primary function of cellular respiration is to break down ATP and release energy

All True orFalse

6.___ During the process of human fermentation the pyruvatemolecule produced at the end of glycolysis is converted into athree carbon molecule called ethyl alcohol while NAD+ is reduced toNADH and a CO2 molecule is released to the atmosphere.

7.___ The proton pumps of the inner membrane of themitochondrion are examples of integral transmembrane,active-transport carrier proteins.

8.___ Photosynthetic pigment molecules of plants are able torelease electrons to an ETC that are utilized to power the eventsof chemiosmosis.

9.__ Photoautotrophs (plants) use inorganic CO2 molecules astheir carbon source for building organic molecules and they usepigment molecules as their proximate source of electrons for theprocess of chemiosmosis.

10.__ The overall process of the TCA/CAC is exergonic with theproduction of ATP molecules and NADH/FADH2 all having stored energyderived from the reactants.

11.__ If a chemical reaction is catalyzed by an enzyme then thereactants will be able to initiate a chemical reaction withslightly more activation energy and the reaction would beclassified as an endergonic process if the products have lessenergy than the reactants.

12.__ The chemical reactions of glycolysis, the Krebs Cycle (TCAcycle), and oxidative phosphorylation, are all processes that canonly be performed when O2 is present (aerobic conditions).

13.___ Photophosphorylation is a type of chemiosmotic processand it possesses a key component of electron transport a finalelectron acceptor and in this case an organic molecule (FEA).

14.___ The pigment molecules, that make up a component of thechloroplasts of a plant, absorb light best in the green wavelengthsand that is why the plants appear so green.

15.___ Features in common for oxidative phosphorylation andphotophosphorylation are an electron source from dinucleotideelectron carrier molecules and an inorganic final electronacceptor.

16.___ In the metabolic pathways we discussed one would expectto find a number of points where allosteric enzymes are associatedwith positive feedback and/or negative feedback systems, and insome systems the allosteric enzyme is affected by both types ofregulatory molecules.

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BIOLOGY 207 Lecture Notes - Lecture 34: Light-Independent Reactions, American Society For Microbiology, Cytochrome

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