BCHS 3304 Lecture Notes - Lecture 25: Bacterial Outer Membrane, Alpha Helix, Beta Barrel
17 Nov 2016
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Peripheral protein= not as tightly associated with membrane, associated to outside of membrane: not insie. Integral proteins- tightly bound to the membrane: span the entire membrane. Transport, regulating flow, receptor, adhesion protein: membrane can be used as anchor to fix position. Membrane separates inside and outside of cell. The outside of cell- protein has carbohydrates bound to it: can be attached via o-glycosilation to ser, thr, The inside of cell- protein has no carbohydrates bound to it. Inside of the membrane- about 19 residues: made of amphiphiles that are oriented asymmetrically. Transmembrane portion can be identified by stretch of forms an alpha helix hydrophobic residues. 19 residues long (cid:498)transmembrane domain(cid:499: hydrophobicity plot= sum of free energy of transfer for 7 residues. The stretch of hydrophobic 19 tm residues: between 5 and 6 turns in alpha helix, length of this alpha helix is about 25.
