BCHS 3304 Lecture Notes - Lecture 11: Asparagine, Protein Disulfide-Isomerase, Phenylalanine
8 Oct 2016
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Document Summary
Tertiary protein structure= the folding of secondary structural elements: can be determined by x-ray crystallography and nuclear magnetic resonance spectroscopy. Can find 3d structures of proteins on online protein data bank (pdb: mass spectroscopy can not be used to determine a protein"s 3-d structure, can contain a motif: a supersecondary structure. Beta- alpha- beta= alpha helix sandwiched between two beta pleated sheets. Beta hairpin= 3 beta sheets with hairpin turn in between them. Greek key= 4 beta pleated sheets folded into a greek key form: protein classification: Beta= only consists of beta pleated sheets. Alpha/ beta= contains both types of secondary structures: large polypeptides domains. Ex: gapdh: n terminus binds to nad+, c terminus binds to gap: secondary structure motif domain protein. Motif and domain are not always present. X-ray crystallography= x rays are bounced off of proteins: electrons in the molecules (atoms and bonds) diffract the x rays, diffraction pattern electron density map.
