CBIO 3400 Lecture Notes - Lecture 7: Eukaryotic Small Ribosomal Subunit (40S), Frameshift Mutation, Missense Mutation
8 Apr 2019
School
Department
Course
Professor
Document Summary
The final step in the central dogma: dna-rna-protein www. dnalc. org. Basics of mrna translation: large and small subunits of ribosomal. The ribosome machine: its rrnas and their processing: pulse chase experiments allow you to follow the processing of components in the cell, dump in radioactive uridine which is only incorporated with rna. This allows you to see where the radioactive piece goes, etc. The triplet code- a look at the message: 3 potential reading frames in all mrnas, only one reading frame is used for translation, where we start determines the frame. Where we start is critical: triplet codons are read in a non-overlapping, comma-less manner. Universal code: more than one codon can specify the same aa, the code is said to be degenerate. The decoder: trnas and their structure: be familiar with the structure of the trna, 3" end is where attach aa, clover leaf structure, base paring to generate the structure, anti-codon: what decodes the message.
