CBIO 3400 Lecture Notes - Lecture 6: Transferase, Eukaryotic Translation, 3D Cell Culture
7 Apr 2019
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Document Summary
Regulation starts before the message is completed: rna processing enzymes associate with phosphorylated ctd tail of rna pol ii (loaded on in stages, some in initiation, elongation, and termination, capping factors, splicing factors, 3" end processing proteins. **5" 7-methyl-g cap is a gtp that"s been added from its 5" carbon to 5" carbon on nascent mrna. Functions of 5" 7-methyl-g cap: prevents rna degradation, stops nuclease from being able to recognize this, facilitates translation, cap serves as binding platform for translation machinery. Splicing and the split or discontinuous gene hypothesis: take double stranded dna and melt it. Then take piece of rna and anneal it with dna and it would keep an r-loop open. It would keep dna from annealing back: what is this overhang about, went on to look at adenovirus genome and isolating rnas; taking single stranded dna and annelid rna to it.
