Key concept: regardless of the purpose of the work all genomic sequencing projects can be broken down into three phases. data collection (getting all the sequences) data analysis (putting sequences in order) February 13, 2014 issue ethical issues with distrubing native american remains. 4 this skeleton of an adult, less than 1,000 ya recent burial; no soft tissues, only bones and teeth. Insert genomic dna at this point is very stringy, like snot. Dna molecules are really long >need to break it up: nebulizer: forces dna under high p from narrow aperture shears the dna, sonicator: blast with high freq sound waves to break. 3" 5" attach linker dna synthesize double-stranded known linker dnas by using ligase. Ligate synthetic known pieces of dna as starting mateiral. Dna will move and fragment based on its length. Recover dna from the block of agarose want this size (good for machien)
