BISC401 Lecture Notes - Lecture 8: Chromosome, Fusion Protein, Tubulin
22 Dec 2016
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Defective nf1 could cause abnormally high ras signaling leading to excessive cell division and formation of the tumors. Multiple classes of noncoding rnas: have lots of functions, transcriptional control/operation, etc. Manipulating dna ligation of dna fragments by dna ligase: dna cut by restriction enzymes can be ligated to other dna sequences by an enzyme called dna ligase. Dna vectors allow transfer and cloning of dna molecules: shuttle vectors: plasmids that can replicate in more than one host organism, e. g. , bacteria. Cloning and amplification of specific dna in bacteria: double-stranded recombinant plasmid dna introduced into bacterial cell, cell culture produces hundreds of millions of new bacteria, many copies of purified plasmid isolated from lysed bacterial cells. Manipulating dna transient and stable transfections: transient transfection, vector is not integrated into the genome of host cells, and therefore can be lost during cell division.
