BISC300 Lecture Notes - Lecture 18: Integron, Lysogen, Proline
27 Feb 2018
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7:19 pm: purple= things said in class, not on slides. Describe bacterial conjugation of the f factor and hfr conjugation. Describe how drug resistance genes arise and are transferred between organisms. "mini chromosomes" can exist independently from host chromosome. Borrow cell"s dna polymerase for replication - don"t have to carry their own can integrate reversibly into the host chromosome (episomes) Most plasmids for genetic engineering do not integrate. Conjugative plasmids (f plasmid) can transfer copies of themselves to other bacteria during conjugation. Encode machinery that enable plasmid to be conjugated from cell to cell. Transfer of plasmid dna from cell with copy of plasmid to cell without. Has the capability to spread from one to another cell. Bacterial conjugation: lederberg and e. tatum demonstrated the transfer of genes between bacteria that depends on direct cell to cell contact mediated by the f pilus unidirectional dna transfer from donor to recipient. Cells that could transfer information have a pillus.
