BISC300 Lecture Notes - Lecture 15: Auxotrophy, Viral Vector, Replica Plating

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Spontaneous--a mutation that arises naturally and not as a result of exposure to mutagens; this is natural. Induced--a mutation caused by exposure to a mutagen radiation: horizontal gene transfer. Transformation--genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane (due to uptake) Transduction--process by which foreign dna is introduced into a cell by a virus or viral vector (due to infection viral) Conjugation--process by which one bacterium transfers genetic material to another through direct contact. During conjugation, one bacterium serves as the donor of the genetic material, and the other serves as the recipient. Mutations: their chem basis and effects: stable, heritable changes in sequence of bases in dna. From alt of single pairs of nucleotides. From addition or deletion of nucleotide pairs. Type of mutations: point mutations transversion or transition.

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