BISC207 Lecture Notes - Lecture 7: Oxidative Phosphorylation, Intermembrane Space, Lactic Acid

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blackpig577

28 Feb 2016

School

University of Delaware

Department

Biological Sciences

Course

BISC207

Professor

Noble- Harvey Jane

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Release of energy to do the work of the cell. Stages: glycolysis (cytoplasm) breaking down of sugars carried out by nearly every cell in the world. Oxidation: loss of electrons/decrease in electron density. Carbon atoms are oxidized and oxygen atom in water is reduced. Energy must be carried by a molecule (usually atp) Electrons are carried with electron carriers (ex: nadh) 2 atp used during phase 1 and 4 atp made in phase 3 (gain of 2 atp) The space between the outer and inner membranes is the intermembrane space. The space inside the inner membrane is the mitochondrial matrix. Donation of electrons from nadh to become nad+, fadh2 becomes fad: nadh = complex 1, fadh2 = complex 2. Electron flow through the chain to the terminal electron acceptor oxygen. When electrons are donated to complex 1, a hydrogen is transported through complex 1 to the intermembrane space.

Related Questions

Where does each of these processes take place within the cell? Possible answers: cytosol, mitochondrial matrix, lysosome, nucleus, inner mitochondrial membrane, outer mitochondrial membrane, mitochondrial intermembrane space

1. Glycolysis cytosol

2. citric acid cycle Mitochondrial Matrix

3. conversion of pyruvate to activated acetyl groups

4. oxidation of fatty acids to acetyl CoA

5. glycogen breakdown

6. release of fatty acids from triacylglycerols

7. oxidative phosphorylation

bronzeimpala88

1.) Certain complexes of the mitochondrial electron transport chain pump protons. Which of the following BEST describes the movement of protons in this situation?

a.) across the outer mitochondrial membrane, from the cytoplasm to the intermembrane space

b.) across the outer mitochondrial membrane, from the intermembrane space to the cytoplasm

c.) across the inner mitochondrial membrane, from the intermembrane space to the matrix

d.) across the inner mitochondrial membrane, from the matrix to the inter membrane space

2.) Which regulatory mechanism is activated when the overall energy availability of a cell is high?

a.) Citrate inhibits phosphofructokinase-1.

b.) ADP up-regulates phosphofructokinase-1.

c.) ATP inhibits phosphofructokinase-1.

d.) AMP up-regulates phosphofructokinase-1.

3.) Electron carriers are oxidized during which of the following reactions?

a.) glycolysis

b.) citric acid cycle

c.) electron transport chain

d.) the conversion of pyruvate to acetyl coA

e.) all of the above

purpleswan410

I have answered 1.) 92 2.)30 3.)? 4.) 12.5 5.) ? Any help wouldbe awesome

Glycolysis, ETC, and Krebs cycle biochemistry product questions:(This is biochemistry)

1. If you created an artificial mitochondria and injected 1molecule of pyruvate inside it, how many protons would accumulatein the intermembrane space of the mitochondria (starting from 1molecule of pyruvate take it all the way to the end of the electrontransport chain). 46 92 104 32

2. If you have created an artificial mitochondria and areinjecting ONE molecule of Pyruvate inside it, how many molecules ofATP can be theoretically generated starting from 1 molecule ofPyruvate ( start from pyruvate and complete all the reactions tofinish oxidative phosphorylation). Remember you also need to takeinto account any ATP generated via substrate Level phosphorylation.11.5 12.5 32 30

3.) If you have generated an artificial mitochondria and haveinjected 1 molecule of Acetyl CoA inside it, how many protons cantheoretically accumulate in the intermembrane space of themitochondria at the end of electron transport chain? 46 32 4636

4.) If you have generated an artificial mitochondria and haveinjected 1 molecule of Acetyl CoA inside it, how many ATP moleculescan theoretically accumulate in the intermembrane space of themitochondria at the end of electron transport chain and oxidativephosphorylation? ( Remember to take into accound ATP moleculegenerated via Substrate Level Phosphorylation) 12.5 9 10 32

5.) You have an artificial mitochondria, and you inject into it10 molecules of a compound X that can donate a pair of electrons tocomplex III of the mitochondria, how many protons can theoreticallyaccumulate in the intermembrane space of the mitochondria ? 60 100104 112

taupeskunk50

BISC207 Lecture Notes - Lecture 7: Oxidative Phosphorylation, Intermembrane Space, Lactic Acid

BISC207 Full Course Notes

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